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publicationDate 2013-04-04-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2013046226-A2
titleOfInvention A novel quantification method for vaccines
abstract To provide a novel and efficient method for estimation of free polysaccharides in multivalent polysaccharide protein conjugate vaccines, wherein anti-carrier protein antibody coupled beads are mixed with sample containing multivalent protein polysaccharide conjugates to enable the separation of conjugated and unconjugated polysaccharide followed by estimation of free polysaccharide content from supernatant by multiplex competitive inhibition assay. The instant method overcomes the limitations of HPSEC & Deoxycholate based methods for free polysaccharide estimation in multivalent polysaccharide protein conjugate vaccines. Additionally total polysaccharide content and percent adsorption of polysaccharides can also be measured by instant method.
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