http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2013013585-A1

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filingDate 2012-07-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7fa8329a84e1270b1b031e7bdb7b6bf9
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publicationDate 2013-01-31-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2013013585-A1
titleOfInvention Thifensulfuron-methyl hydrolysis enzyme gene tsme and use thereof
abstract Provided are a thifensulfuron-methyl hydrolysis enzyme gene tsmE and use thereof. The nucleotide sequence of the thifensulfuron-methyl hydrolysis enzyme gene tsmE is SEQ ID NO:1, with a full length of 1194bp, and a G+C content of 51.09%; the thifensulfuron-methyl hydrolysis enzyme gene tsmE encodes a polypeptide with the full length of 389 amino acids, and the amino acid sequence is SEQ ID NO:2. The thifensulfuron-methyl hydrolysis enzyme gene tsmE provided can completely degrade 100mg/L of thifensulfuron-methy within an hour, and hydrolyze the thifensulfuron-methy into a thifensulfuron-sulfonic acid with no herbicidal activity; furthermore, the tsmE can also completely degrade 100mg/L of herbicide Gallant-Super within an hour. Also provided is a use of the thifensulfuron-methyl hydrolysis enzyme gene tsmE for constructing an anti-thifensulfuron-methyl transgenic crop, and a use of the thifensulfuron-methyl hydrolysis enzyme tsmE for degrading the thifensulfuron-methyl and the Gallant-Super.
priorityDate 2011-07-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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