| abstract |
The purpose of the present invention is to provide a nucleic acid analysis method which has such a completeness that 1,000 or more types of nucleic acids can be analyzed in one time, also has such quantitative performance that the dynamic range is four more figures, and is simple, particularly provide a nucleic acid analysis method in which 10,000 or less types of nucleic acids can be analyzed and is extremely effective for the analysis of non-translated RNA or micro RNA.nAccording to the present invention, one molecule of each of nucleic acid fragments to be analyzed is provided, a nucleic acid molecule having a known nucleotide sequence and labeled fluorescently is hybridized with each of the nucleic acid fragments to be analyzed, and subsequently a phosphor bound to the hybridized nucleic acid molecule as a label is detected. In this manner, the analysis of nucleic acids can be carried out in a simple manner and rapidly without employing any amplification reaction such as a PCR, at good completeness and quantitative performance with respect to the types and abundances of the nucleic acids to be analyzed, and at a sensitivity and a resolution capacity of single molecule levels. |