| abstract |
The present invention relates to a probe for iFRET and to a usage of same. More particularly, the present invention relates to the probe for iFRET, a method for manufacturing the probe for iFRET, a method for searching a target protein-specific binding site or a molecule having the binding site using the probe for iFRET, and a method for imaging the target protein through the probe for iFRET. The probe for iFRET, according to the present invention, unlike the existing FRET method, uses an amino acid inside a protein as a fluorescence donor, thereby using only one fluorescent substance, and has an light emitting wavelength which is separated from a protein intrinsic fluorescence, thereby having high specificity and sensitivity and thus allowing easier and more accurate analysis of the amount, activity, and mechanism, among others, of a variety of proteins. |