http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2012176016-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_08974b981a18409579f5587f01300000 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1f179e34a28ae163c5f18b68229d8b58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_60de65563c6d5214513dd4c8704e826c |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-905 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-81 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 |
| filingDate | 2011-06-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ae97879a869f3c992b82f75f638e0495 |
| publicationDate | 2012-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2012176016-A1 |
| titleOfInvention | Cassettes and methods for transforming and selecting yeast transformants by homologous recombination |
| abstract | The invention relates to a method for selecting a transformed yeast cell having integrated a nucleic acid fragment of interest by homologous recombination said method comprising the steps of: (i) Contacting a yeast cell with: The vector comprising: a positive selection gene, o a homologous recombination site comprising a 51 and a 31 recombination regions framing a restriction site, and A nucleic acid fragment of interest to insert by homologous recombination into the homologous recombination site of said vector, said nucleic acid fragment being flanked by regions substantially identical to the 51 and 31 recombination regions of the homologous recombination site, and (ii) Transforming said yeast cell with said vector and said nucleic acid fragment of interest, (iii) Selecting yeast cells harboring said vector with said positive selection gene, Characterized in that: A negative selection gene is further present in the vector downstream to the homologous recombination site and under the control of a promoter situated upstream to said homologous recombination site, said promoter and negative selection gene being operably linked in said vector before insertion of the DNA fragment of interest, and 25 (iv) The method further comprises a step of selecting yeast cells harboring the DNA fragment of interest using the negative selection gene. The invention further provides cassettes and kits for carrying out the method of the invention. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3088535-A1 |
| priorityDate | 2011-06-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 916.