| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7c2abd09a6e8c8d6bab441582746deba
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7766064ebf0f1e9534be7d6a239867fd
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_74e1c7f3a6608121e120e6cc430a7986
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d03a23980e558bd5501fb56d07060b56
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0c37ea6c0ccf659261758e2450ac848e |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-14 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0606
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0607
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0662
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0663 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0775
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0735 |
| filingDate |
2012-05-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1535310c8ce3b3f5f81da3de31e7a93d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7ad759e315020fa8009b3d2db99adfd1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6477677c90c3e653646ad63970088663
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6743bfb0d563a83b7d9019312e1373d0 |
| publicationDate |
2012-11-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2012158899-A1 |
| titleOfInvention |
Passaging and harvesting formulation and method for human pluripotent stem cells |
| abstract |
Formulations and methods are disclosed for the harvesting and subsequent passaging of human pluripotent stem cells without the use of enzymes and/or scraping to dislodge cells from cell culture vessels. The formulations and methods permit the harvesting of cells as large clusters from the surface of various cell culture vessels including multilayer cell culture vessels. Further, the formulations and methods provide high yields of harvested cells for subsequent passaging and high post-harvest cell viability. Pluripotent stem cells passaged with the formulations according to the methods remain undifferentiated and express typical stem cell markers, while, at the same time, they retain the differentiation capability and are able to differentiate into the cells in all three germ layers and generate teratomas, even after numerous rounds of harvesting and passaging. These hPSCs also maintain normal karyotype after passaged with the formulations for extended period of time. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021116362-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-WO2014196549-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11058106-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2018136500-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20190104407-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2017515510-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-102580997-B1 |
| priorityDate |
2011-05-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |