| abstract |
A delivery system and preparation of siRNA, with nanoparticles enclosing siRNA as an active ingredient, wherein the nano-particles are formed by a polymer and a cationic lipid, the polymer is a di-block or tri-block copolymer of polyethylene glycol-polylactic acid or polyethylene glycol-poly(lactic acid-glycolic acid), the cationic lipid is N,N-dihydroxyethyl-N-methyl-N-2-(cholesteroloxy carbonylamino) ethylammonium bromide or (2,3-dioleyloxy propyl) trimethylammonium chloride. The nanoparticle and preparation can desirably deliver siRNA to a cell and effectively silence expression of a target gene. Moreover, after being modified by a ligand or antibody, the siRNA delivery system can desirably silence the target gene at both a cellular level and an animal level. |