| abstract |
In an adipocyte culture method, seeded adipocytes are cultured while adhering the adipocytes to a culture bottom surface (14) and walls (12) positioned perpendicularly to the culture bottom surface (14), and mature adipocytes are obtained by generating spherical, enlarged fat droplets in cells without suspending the adipocytes in a culture medium. In the method, a culture vessel (10) is used in which the culture walls (12) are formed perpendicularly to the culture bottom surface (14). With cells or cell aggregates adhered in at least two places, namely the walls (12) and the culture bottom surface (14), spherical mature adipocytes having spherical enlarged fat droplets in cells are cultured. Adipocytes with a form close to in vivo adipocytes are thus obtained. |