http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2012119546-A3

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filingDate 2012-03-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2012-11-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2012119546-A3
titleOfInvention Method for preparing recombinant escherichia coli to produce succinic acid through fermentation
abstract A method for preparing recombinant Escherichia coli to produce succinic acid through fermentation. The method mainly involves increasing the synthesis rate of succinic acid by overexpressing NAD+ biosynthesis-related genes in Escherichia coli, and adding a certain concentration of nicotinic acid to the anaerobic culture medium as the precursor material of NAD+ biosynthesis, thus increasing the strain growth rate in anaerobic conditions and the synthesis rate of succinic acid.
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