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filingDate 2012-02-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_461ebd32d2bc6b816fd36f775ddbe43a
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publicationDate 2012-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2012114227-A1
titleOfInvention Biosensor and method of manufacturing such a biosensor
abstract The invention relates to a method of manufacturing a biosensor. The biosensor of the invention comprises a microbeam, which is the mobile part of the biosensor, linked to a support, at least one electrode, at least part of which is embedded in the microbeam, at least one biological molecule A grafted onto the microbeam in a different zone from the zone wherein the at least one electrode is embedded, and a mechanoelectrical transducer for converting variations of the mechanical properties of the microbeam into an electrical signal, when the biological molecule A is placed in contact with a biological molecule B to be detected and/or quantified. The method of the invention is characterized in that the microbeam and its support are made of a fluoropolymer material and form an integral component, and in that it comprises the following steps: a) formation of at least one electrode on fluoropolymer material sheet, b) optionally formation of a ferromagnetic material pad on the electrode(s), c) passivation of the electrode(s), d) creation of the final desired form of the biosensor in the sheet of polymer material and separation of this form from the sheet, e) optionally prefunctionalization of a zone of the microbeam, this zone being different from the zone wherein the at least one electrode is embedded, f) functionalization either of the prefunctionalized zone when step e) is implemented, or of a zone of the microbeam, this zone being different from the zone wherein the at least one electrode is embedded, and g) grafting of at least one biological molecule A onto the functionalized zone obtained in step f). The invention finds application, in particular, in the field of the detection of biological molecules.
priorityDate 2011-02-22-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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