http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2012109642-A1

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publicationDate 2012-08-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2012109642-A1
titleOfInvention Aqueous extraction methods for high lipid microorganisms
abstract Systems and methods for obtaining an extracted lipid fraction from microorganisms are described. The method includes growing the microorganisms in aqueous culture under conditions to provide microorganisms having a high level of lipids. The culture of microorganisms is then dewatered to provide a concentrated culture having an increased amount of solids by weight while still including a substantial amount of water. A controlled disruption of the concentrated culture is then carried out to provide a disrupted culture, which is then separated under aqueous conditions to provide an extracted lipid fraction.
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