abstract |
A fusion protein of the present invention comprises an endoplasmic reticulum calcium ATPase, a fluorescence donor for FRET and a fluorescence acceptor for FRET. One of the fluorescence donor and the fluorescence acceptor is connected to the N-terminus of the ATPase, and the other is inserted between the one and the ATPase or inserted into an amino acid sequence in the ATPase corresponding to the first to sixth amino acids, the 369th to 380th amino acids or the 572nd to 583rd amino acids of SERCA2a. |