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filingDate 2011-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7982dc3d333f6007319ceb2d266413ac
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publicationDate 2012-07-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2012094934-A1
titleOfInvention Method for determining pectin content in plant sample
abstract Disclosed is a method for determining pectin content in a plant sample, comprising the following steps: 1) adding an acidic alcohol solution to the plant sample, then heating the resulting mixture to reflux in a water bath, followed by a first filtration; 2) soaking the filtered residue obtained from the first filtration with an acidic solution, then heating the resulting mixture to reflux in a water bath, followed by a second filtration, after cooling bring to volume, obtaining filtrate for later use; 3) adding an acetic acid/sodium acetate buffer solution to treat the filtered residue obtained from the second filtration, then adding a pectinase solution and heating the resulting mixture under vibration in a water bath, followed by a third filtration to obtain a filtrate for later use; 4) sequentially adding an acetic acid/sodium acetate buffer solution and a pectinase solution to the filtrate obtained in step 2) and heating the resulting mixture under vibration in a water bath to obtain an enzymatic hydrolysate, then adding the filtrate obtained in step 3) to the enzymatic hydrolysate followed by bringing to volume, obtaining a test solution; and 5) drawing the test solution into a continuous flow analyzer to perform analysis. The method disclosed has the advantage of providing accurate analysis results.
priorityDate 2011-01-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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