| abstract |
The present invention relates to a bivalent binding agent capable of binding a polypeptide dimer the binding agent consisting of two monovalent binders that are linked to each other via a linker, wherein the first monovalent binder binds to an epitope of a first target polypeptide comprised in said dimer, wherein the second monovalent binder binds to an epitope of a second target polypeptide comprised in said dimer, wherein each monovalent binder has a Kdiss in the range of 5x10 -3 /sec to 10 -4 /sec, and wherein the bivalent binding agent has a Kdiss of 3x10 -5 /sec or less. A method of making such bivalent binding agent and the use of such bivalent agent in histological staining procedures are also disclosed. |