| abstract |
Concurrent multi-region static or dynamic imaging of different laterally or depth-displaced, same or different shaped regions of a tissue or other sample can be performed, such as by using spectral encoding and decoding, which can optionally be combined with temporal encoding and decoding. Detected response light emitted from the sample can include a fluorescence response, or a scattering response such as one including a higher order harmonic such as Second Harmonic Generation (SHG) or Third Harmonic Generation (THG), or an enhanced Raman signal such as one acquired using coherent anti-Stokes Raman Scattering (CARS) or stimulated Raman scattering (SRS) microscopy. The imaging technique can allow different concurrent responses to be concurrently observed, such as surface blood flow and deeper intracellular calcium changes in a brain tissue sample, blood flow at multiple layers of the brain, or calcium signals at multiple layers. |