| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9a21480b229e14ce0a512307bb84199f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_febd6f596c2795259b2b13f95b26a94c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fd34ffa3d2165f22d2e41e3d3089d7c6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_44f64564fd3d3aac41767e8c87cbeec8 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07C233-54
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07C229-36 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-04 |
| filingDate |
2011-09-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_af84ecaeef89298048bd18b23f6c4c11
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_46916c786d03252ea051fcd3aa80138c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0466582572a34e687c3eacad28877f91 |
| publicationDate |
2012-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2012034043-A1 |
| titleOfInvention |
Genetically encoded initiator for polymer growth from proteins |
| abstract |
This invention pertains to methods for producing homogeneous recombinant proteins that contain polymer initiators at defined sites. The unnatural amino acid, 4-(2'-bromoisobutyramido)phenylalanine of formula 1, was designed and synthesized as a molecule comprising a functional group further comprising an initiator for an atom-transfer radical polymerization (ATRP") that additionally would provide a stable linkage between the protein and growing polymer. We evolved a Methanococcus jannaschii (Mj) tyrosyl-tRNA synthetase/tRNAcuA pair to genetically encode this unnatural amino acid in response to an amber codon. To demonstrate the utility of this functional amino acid, we produced Green Fluorescent Protein with the unnatural amino acid initiator of formula 1 site- specifically incorporated on its surface (GFP-1). Purified GFP-1 was then used as an initiator under standard ATRP conditions with oligo(ethylene oxide) mononiethyl ether methacrylate, efficiently producing a polymer-GFP bioconjugate wherein the polymer is connected at a specifically selected site on GFP. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11174325-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9790305-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8816001-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9982070-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9644042-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3003342-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9243274-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9533297-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10072042-B2 |
| priorityDate |
2010-09-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |