| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_843bd9e7c95bf0fea2effbfa287c06f5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_66467fe5b0acf469afb972f300bcfb87
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fc6e7f6c096e081d02e97463be7c8298 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-2311
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2506-03
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0618
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0662
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-90
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-237
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-2306
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-23
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-13
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-115
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-92
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-98
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2506-45
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2506-02 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0618
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0606
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0607
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0653
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0655
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0662
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0696
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0654 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0735 |
| filingDate |
2011-09-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_21d460aa69ef7b8cfb18740484949969
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_569905f999e71b11f5d611759e569b09 |
| publicationDate |
2012-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2012032521-A2 |
| titleOfInvention |
Novel methods and culture media for culturing pluripotent stem cells |
| abstract |
Provided is an isolated population of human pluripotent stem cells comprising at least 50% human pluripotent stem cells characterized by an OCT4 + /TRA1-60-/TRA1-81 - /SSEA1 + /SSEA4 - expression signature, and novel methods of generating and maintaining same in a pluripotent, undifferentiated state a suspension culture devoid of cell clumps. Also provided are novel culture media for culturing pluripotent stem cells such as embryonic stem cells and induced pluripotent stem cells in a suspension culture or a two-dimensional culture system while maintaining the cells in a proliferative, pluripotent and undifferentiated state. The novel culture media comprise interleukin 11 (IL11) and Ciliay Neurotrophic Factor (CNTF); bFGF at a concentration of at least 50 ng/ml and an IL6RIL6 chimera; or an animal contaminant-free serum replacement and an IL6RIL6 chimera. Also provided are cell cultures comprising the pluripotent stem cells and the culture media and methods of using same for expanding and maintaining pluripotent stem cells in an undifferentiated state, or for generating lineage-specific cells from the pluripotent stem cells. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10597635-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10214722-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11661584-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2015181253-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022259254-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-101855967-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11193108-B2 |
| priorityDate |
2010-09-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |