| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_29f45b509e769fa10c28d723f1f77c1c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_efda7341819f1a85d7f2fe104cd8a26f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c3a3383979f5c52e71c22bd98d0f8b01
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7851f2dbddbc846a5b444c96aa46a4b6 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1017
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1006 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate |
2011-09-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e23899affb0510b13b5c789f3f7352f9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ff0b59dd60a7d3b3ce3ae143fe9ee856
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7e46c3e2c0a4c2257eddc40ed9c8afa0 |
| publicationDate |
2012-03-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2012028737-A1 |
| titleOfInvention |
Method for isolating a target nucleic acid including small target nucleic acids with high yield |
| abstract |
The present invention pertains to a method for isolating a target nucleic acid including small target nucleic acids from a sample, said method comprising at least the following steps a) binding at least a portion of the target nucleic acid including small target nucleic acids to a nucleic acid binding solid phase comprised in a column by passing the sample through said column, b) performing an enzymatic and/or chemical treatment on the nucleic acid binding solid phase while the target nucleic acid is bound to said solid phase, c) collecting at least a portion of the small target nucleic acids released from the solid phase during said treatment of step b) as flow-through, d) contacting said flow-through which comprises small target nucleic acids mixed with a recovery solution with a nucleic acid binding solid phase for binding the contained small target nucleic acids to said nucleic acid binding solid phase, e) optionally performing an elution. The present invention results in a considerable increase in the yield of small target nucleic acids in the isolated target nucleic acid because it allows to efficiently capture and recover small target nucleic acids. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10329553-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10260063-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2019063071-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2015157650-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2018064562-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2015526100-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2014033326-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106164270-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10584327-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104603267-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3209403-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3133159-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104603267-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-2554572-B |
| priorityDate |
2010-09-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |