Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_15e351bb60d4621c95e7f3ecb2f9a2bd http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0c16430e4cc12546a427615b2d4c131e http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_db628fbc812ea146bb6eb24a8c0ab43a |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-80 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6813 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6804 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-2863 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1055 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6845 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6842 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-00 |
filingDate |
2011-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_38dee32f1894cea97a040f595c51a6dc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_846131179e9a1f540d65afca45df4fdf |
publicationDate |
2011-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
WO-2011161420-A2 |
titleOfInvention |
Coincidence detection |
abstract |
The invention relates to a method of detecting the coincidence of two biomolecular structures in a solid phase sample, said method comprising: (i) providing a first and a second fusion protein, each fusion protein comprising (a) a detection domain, said detection domain comprising a DNA binding domain; said detection domain capable of binding a cognate specific nucleotide sequence in co-operation with a further detection domain; (b) a recognition domain, said recognition domain capable of binding a target biomolecular structure; and (c) a connector domain; said connector domain being fused at one end to the detection domain and being fused at the other end to the recognition domain; wherein at least two of (a), (b) and (c) are heterologous to one another; wherein the recognition domains of said first and said second fusion proteins are capable of binding to first and second biomolecular structures; (ii) contacting the sample with said first and second fusion proteins; (iii) incubating to allow binding; (iv) removing unbound fusion protein; (v) contacting the sample with nucleic acid comprising said cognate specific nucleotide sequence; (vi) incubating to allow heterotrimeric binding of the nucleic acid; (vii) detecting nucleic acid bound to the sample wherein detection of nucleic acid in step; (vii) indicates that the two biomolecular structures are present coincidentally in said sample. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113125747-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113125747-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9335292-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2015052335-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-2562721-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10852274-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11505799-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106279419-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11560565-B2 |
priorityDate |
2010-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |