| abstract |
The present invention relates to modified α-gliadins, comprising the three T cell epitopes DQ2-Glia-α1, DQ2-Glia-α2 and DQ2-Glia-α3, wherein the amino acid in position 3 and/or 8 of the DQ2-Glia-α1 epitope P 1 { F/Y} 2 P 3 Q 4 P 5 {Q/E} 6 L 7 P 8 Y 9 is replaced by another amino acid, preferably serine, the amino acid in position 3 and/or 8 of the DQ2-Glia-α2 epitope { P/F} 1 Q 2 P 3 {Q/E} 4 L 5 P 6 Y 7 P 8 Q 9 is replaced by another amino acid, preferably serine, and the amino acid in position 3 and/or 8 of the DQ2-Glia-α3 epitope F 1 R 2 P 3 Q 4 Q 5 P 6 Y 7 P 8 Q 9 is replaced by another amino acid, preferably serine. In addition to this the amino acid in position 7 of all three epitopes may be replaced by another amino acid. The invention further relates to plants that comprise in their genome, a polynucleotide encoding the modified α-gliadins, and to grains and food products that comprise the α-gliadins. |