http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2011150754-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e383de964c880698f8d78033f53ac2aa http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_01c59a0511fb06fac09a7d8897e7ca12 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2402 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01108 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-645 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-38 |
| filingDate | 2011-05-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d10ae74416193206ecc78ecc916f73bd |
| publicationDate | 2011-12-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2011150754-A1 |
| titleOfInvention | Method for preparing and extracting extracellular lactase |
| abstract | A method for preparing and extracting extracellular lactase is provided, which comprises the following steps: (1) inoculating activated Actinomucor elegans strain into a solid culture medium to culture; (2) adding inorganic flocculants into cultured crude enzyme solution to precipitate it, then filtering to obtain a semi-finished product; (3) the obtained semi-finished product is salted out by ammonium sulfate grading sedimentation method and purified by Sephadex G-100 gel chromatography and diethylaminoethyl cellulose (DEAE)-cellulose-52 ion exchange chromatography to obtain the lactase finished product. Compared with the method for producing lactase intracellularly in the art, the method of the present application is simple and suitable for large scale manufacturing of extracellular lactase, and provides extracellular lactase with high heat resistance and good stability. And the working pH of the present method is suitable for the internal biological environment of the organism. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AU-2015356272-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3228704-A4 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10368558-B2 |
| priorityDate | 2010-05-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
| Predicate | Subject |
|---|---|
| isDiscussedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID8471 http://rdf.ncbi.nlm.nih.gov/pubchem/substance/SID128671575 |
Total number of triples: 19.