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filingDate 2011-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d923f7a0664909e73cd2c3ba54b11951
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publicationDate 2011-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2011147563-A1
titleOfInvention Method for electrochemically detecting binding reactions
abstract The invention relates to a method for carrying out homogenous immunoassay formats with highly sensitive electrochemical detection in solution, characterized in that two different conjugates are combined as reagents with the sample or a sample/buffer mixture, wherein one conjugate comprises a redox marker and an analyte molecule and the second conjugate comprises an anti-redox marker antibody or a specifically binding fragment thereof and a molecule that specifically binds the analyte. The method is suitable for wash- and separation-free immunoassays in diagnostics, in particular for automated and microfluidic immunoassays. The method is also suitable for inexpensive immunoassay systems and test strips that are disposable.
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