http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2011142813-A4
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5aab11b560f6ffb4ede42051e84c2e0e http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_38b4c2e07bfcebffa5f00a51c69a55dc http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d0704c01cd1115e9e7311814638fcb19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e232689f4a1fc9ec99d4344c059b061b http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_aec05fbed74d7ef21570d05eefe762cf http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c3b89f8a9549bfb17b87cc8c157c7532 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N13-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M35-02 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-26 |
filingDate | 2011-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4880d94749137f5b1c87f68b0b497999 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1258e7a07191b781e85afb10f0bd4e82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_620b2384834bd0517b008805b9b2f2eb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_15b176c79a848f5186de93c6bd28d991 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_323b88c0de16d16ba1d9fa46a6a06a66 |
publicationDate | 2012-01-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | WO-2011142813-A4 |
titleOfInvention | Dynamic mixing and electroporation chamber and system |
abstract | The present invention relates to an apparatus for mixing cells and exogenous material for delivery of the exogenous material into cells using electroporation in a manner that preserves cell viability and integrity of the exogenous material. The exogenous material can be polynucleotides, peptides, proteins or other pharmaceutical molecules. It further provides a chamber that can be used to electroporate the contents of the chamber after mixing. In accordance with the invention, cell suspensions that may be contaminated with degrading enzymes are stored separately from water-based liquids that contain the exogenous material. Contact of the two water-based liquids (cell suspension and exogenous material) first occurs within the chamber. The chamber of the present invention has two or more inlet ports to allow separate entrance of water-based liquids. Two other ports are vent and exit ports. The inside of the chamber allows flow of all water-based liquids down the same side of the chamber and curved comers for redirecting flow direction and gently mixing water- based liquids. The chamber also includes electrodes as chamber walls for electroporation of chamber contents. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108254216-A |
priorityDate | 2010-05-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Predicate | Subject |
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isDiscussedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/substance/SID419512635 http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID962 |
Total number of triples: 25.