| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8ec2d24282a939840c62289e9171cb3a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_61863d5cb6587e1df9aac262ec397095
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d950e961a09866e2049c7d8d779a81b8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_344fe7b590110ddf0407d202239e9c18
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f26889064a110b72740fff8409f11abb
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bfd614532bb06f13bb7c627160255575 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-02 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-625
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
| filingDate |
2011-03-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d22efb93db29bf2a4178893c63dd02f5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3d44afcf4c539229eed47fda9b7e36ec
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_61ae38a16ea50c25fbe8e1c1d1d7f0df
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9ba320e38709b1cc8b6f14779f0a4851
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_381976b76a654593847611e791806b99 |
| publicationDate |
2011-11-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2011142529-A2 |
| titleOfInvention |
Soluble expression of bulky folded active proteins |
| abstract |
The present invention relates to expression vectors and methods for enhancing soluble expression and secretion of a heterologous protein, particularly a bulky folded active heterologous protein which has one or more transmembrane-like domains or intramolecular disulfide bonds by linking a leader peptide with acidic or basic pI and high hydrophilicity thereto; by substituting one or more amino acids within N-terminal of the heterologous protein with ones having acidic or neutral pI and high hydrophilicity; or reducing elevating G RNA value of a polynucleotide encoding the leader peptide having basic pI value and high hydrophilicity. The expression vector and the method may be used to produce of heterologous protein and to transduce of therapeutic proteins in a patient by preventing formation of insoluble inclusion body and by enhancing secretional efficiency of the heterologous protein into the periplasm or outside cell. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2012137187-A1 |
| priorityDate |
2010-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |