| abstract |
Disclosed is a nucleic acid base analog with quenching characteristics and fluorescence and application thereof. Specifically disclosed is a quencher characterized by having a 2-nitropyrrole structure shown by formula (I). [In formula (I), R 1 and R 2 are groups selected independently from a group formed from ribose and deoxyribose; hydrogen, hydroxyl groups, SH groups, and halogens; optionally substituted C 2-10 alkyl groups, alkenyl groups, and alkynyl groups; one or a plurality of 5-member heterocycles, one or a plurality of 6-member heterocycles, one or a plurality of polycyclic heterocycles and one or a plurality of aromatic rings, containing nitrogen atoms or sulfur atoms; sugars, sugar chains, amino acids, and peptides; and florescent molecules bound through linkers.] |