| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_dbc4f11bd2095af21853384fa75e850c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e4ac146bea423487a20940867430fedc
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_acb2f5e757d4f56b665ed2664ee2503d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f3d4964b5a4fef7e756d169c4a0b9c75
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c93c354eaf65969e544430c293f5fe9e |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6886 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-63 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
| filingDate |
2011-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_befd2c8a2cd348ae83b325216bb3cf7d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_01809ddc91642772033b060ed578e8c5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_91721d4068090899d072d0102159e6ab
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_db18ba994ba336957309c5375db665f5 |
| publicationDate |
2011-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2011131145-A1 |
| titleOfInvention |
Liquid chip for detecting egfr gene mutations |
| abstract |
The invention belongs to biochip technical field and discloses a liquid chip, for detecting EGFR gene mutations, comprising: (i) allele-specific primer extension (ASPE) primer pair(s) designed for both wild-type and mutant EGFR gene, where each ASPE primer comprises a tag sequence at 5' end and a specific primer part at 3' end, which is aiming at the mutant site of target gene; (ii) microspheres coated separately with a specific anti-tag sequence and encoded with different colors, where the said anti-tag sequence matches correspondingly with the said tag sequence; and (iii) amplification primers for amplifying target sequence(s) with mutant site(s) of Exon 19, Exon 20 and /or Exon 21. The detection method provided by the present invention has a coincidence rate up to 100% with sequencing methods. The liquid chip for detecting EGFR gene mutations prepared is excellent in signal to noise ratio, and cross reaction between designed probes and anti-tag sequences is basically free. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2495335-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2495335-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2012065705-A1 |
| priorityDate |
2010-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |