http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2011124684-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_12a270c3d815979731da6df1411e118f http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_497e7fc3bc48d5ffe2e470a68f88689d http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f93b8a5de44029dcd6150a84ded68ca4 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6818 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6486 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2011-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d3a11282c698bd2ef05fd7bab28a6d11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1e6c2150b7d93d862bd62ebc3ecf0dbe |
| publicationDate | 2011-10-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2011124684-A1 |
| titleOfInvention | Method for detecting specific nucleic acid sequences |
| abstract | The present invention relates to a method and test kit for detecting specific nucleic acid sequences, comprising the steps of: 1. matrix-dependent new synthesis of the target nucleic acid; 2. target-specific probe hybridization; and 3. detection of the hybridization event. The invention is characterized in that, in the first step, an oligonucleotide 1, which is marked by a marker 1 and is entirely or partially complementary to the target sequence, acts as a primer in the matrix-dependent new synthesis of the target nucleic acid and, in the second step, an oligonucleotide 2, which is marked by a marker 2 and, owing to its melting temperature being lower than that of the oligonucleotide 1, is not involved in the first step, partially or completely hybridizes with the DNA new synthesis product of oligonucleotide 1. The detection of the hybridization reaction can take place both fluorometrically in the form of a homogeneous assay and, for verification of the result, subsequently immunologically. The detection reaction always takes place in time after the matrix-dependent new synthesis has been carried out. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2569444-A1 |
| priorityDate | 2010-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 128.