abstract |
There is presently provided a method of reducing DNA mismatches in a pool of double-stranded DNA molecules, the pool comprising 5'-protected double-stranded DNA molecules, by treating the pool with a mismatch endonuclease and a DNA ligase, followed by treating the pool with one or more exonucleases, the one or more exonucleases together having 5' to 3' activity on double-stranded DNA and 3' to 5' activity on single-stranded DNA. |