| abstract |
The present invention generally relates to methods of obtaining peripheral blood-derived MSCs (PB- derived MSCs) and bone-marrow derived MSC (BM-derived MSCs) from a peripheral blood sample obtained from a human subject. In particular, the present invention relates to the surprising discovery that the mobilization of MSC into the peripheral blood is temporally regulated and that the number of circulating MSCs in human peripheral blood peaks after at least 2 days post administration of a mobilizing agent, such as G-CSF or GM-CSF to a human subject. Accordingly, in some embodiments, the present invention relates to methods of obtaining PB-derived and BM-derived MSC from peripheral blood from a human subject in vivo and ex vivo using mobilizing agents, such as G-CSF or GM-CSF, where administration of G-CSF and/or GM-CSF are optimized to mobilize MSCs and enrich the number of MSCs in the peripheral blood as apposed to other stem cell populations, such as HSCs. |