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publicationDate 2011-04-21-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2011047090-A1
titleOfInvention Methods of diagnosing and monitoring rejection mediated by antibodies
abstract An intracellular cytokine flow cytometry (CFC) assay was developed to measure CD3- (non-T) cell response to allo-Ags expressed on peripheral blood mononuclear cells (allo-CFC-PBMC) and/or endothelial cells (allo-CFC-EC) by detecting intracellular gamma-interferon (IFN Ƴ ) production. The assay can be used to determine a likelihood of antibody mediated rejection in an individual. A method for using genetic screening to determine the likelihood of AMR is also disclosed.
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