abstract |
Microfluidic devices and methods for using microfluidic devices for immobilizing cells, e.g., mammalian cells, suspended in a flowing fluid include selecting the geometry and dimensions of one or more multi-surface cavities disposed therein, and/or the flow direction of a cell-containing fluid therein. The cavity can comprise an upper and lower surface, and cells can be captured on either surfaces located at two different depths within a cavity. The invention includes methods for generating co-cultures of different cell populations inside a multi-surface cavity disposed within microfluidic devices as disclosed herein. |