abstract |
Systems methods and compositions for generating target sequence-linked solid supports (e.g., 5 beads) using a solid support linked to a plurality of capture sequences and capture primers composed of a 3' target-specific portion and a 5' capture sequence portion are provided. The target sequence linked solid support is used in sequencing methods (e.g., pyrosequencing, zero-mode waveguide type sequencing, nanopore sequencing, etc.) to determine the sequence of a target sequence (e.g., in order to detect the identity of a target nucleic acid in sample). |