abstract |
The invention relates to a method that utilizes nucleic acid deactivating reagents for the deactivation of contaminating nucleic acids, and thermophilic proteinases for the extraction of nucleic acids in a closed-system to be used in tandem with methods for the amplification of target nucleic acids present in a sample. The combined method enables simplified, temperature-controlled devices to be used for accurate, streamlined testing at the point of care for a wide variety of applications in the medical, industrial, environmental, quality control, security and research fields. |