| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1329dbb24facdaa3b141f2d777e4da4d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a951a643aa9713594882ab10fc688b62
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_77682a3c5e8b79053120b3467d6362d9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bcff13f8573db698307f919e6385d5c4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_59536bb359112da08e18bdd319dcdbef
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_45fe161ed46ab0d1fafd6ec2f9dbddec |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L3-502784 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/H01J49-165 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-28 |
| filingDate |
2010-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9022f1f73858208af62e176f3bd2315c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1a790f75cd3802c30b506bf231fe378c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9fd28cf86b03618533462ba1bddd50f6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_479472d8b9689e3dae3abff146c78c56
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6667520a823975852a1c6a001e66955b |
| publicationDate |
2011-04-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2010148339-A3 |
| titleOfInvention |
Electrospray and nanospray ionization of discrete samples in droplet format |
| abstract |
Droplets or plugs within multiphase microfluidic systems have rapidly gained interest as a way to manipulate samples and chemical reactions on the femtoliter to microliter scale. Chemical analysis of the plugs remains a challenge. It has been discovered that nanoliter plugs of sample separated by air or oil can be analyzed by electrospray ionization mass spectrometry when pumped directly into a fused silica nanospray emitter nozzle. Using leu-enkephalin in methanol and 1% acetic acid in water (50:50 v:v) as a model sample, we found carry-over between plugs was < 0.1% and relative standard deviation of signal for a series of plugs was 3%. Detection limits were 1 nM. Sample analysis rates of 0.8 Hz were achieved by pumping 13 nL samples separated by 3 mm long air gaps in a 75 μm inner diameter tube. Analysis rates were limited by the scan time of the ion trap mass spectrometer. The system provides a robust, rapid, and information-rich method for chemical analysis of sample in segmented flow systems. |
| priorityDate |
2009-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |