Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_459549086df37b5bed0003502de7e7aa http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_71300c3d99c2e249a307f35b76b4bec6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9450d9aac786b86a8b6ae4c6832fdc62 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P43-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-535 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-535 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-36 |
filingDate |
2010-06-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dc026c17e0d877203ffe88cafc81f713 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_db833d8a97cb25ce899fc940e860e701 |
publicationDate |
2010-12-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
WO-2010146599-A1 |
titleOfInvention |
Process for purification of recombinant human granulocyte colony stimulating factor |
abstract |
The present invention describes a novel process for large-scale purification of therapeutic grade quality of recombinant human GCSF from microbial cells, wherein the protein is expressed as inclusion bodies. The Inclusion bodies are solubilized and refolded under redox condition. The Redox condition is provided by using ascorbic acid, dehydroascorbic acid and reduced gluthathione. The process involves the novel use of aqueous two phase extraction step to purify refolded GCSF after removal of denaturant. After this step GCSF is further purified using chromatography techniques for removal of related impurities. The GCSF obtained has good purity and yields which are essential for a production scale process. The host cell related contaminants like proteins, DNA and endotoxins are reduced using the purification processes of the invention. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2014030213-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-102011001743-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9416164-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2014155349-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9422354-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9982012-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2020234742-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3517621-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2016031932-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114853872-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9458207-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2013068602-A2 |
priorityDate |
2009-06-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |