| abstract |
Disclosed is a means for inducing the differentiation of a pluripotent stem cell into a thymic epithelial progenitor cell or a medullar thymic epithelial cell. The means comprises carrying out a first induction step of causing the differentiation of a pluripotent stem cell into a definitive endodermal cell and a second induction step of causing the differentiation of the definitive endodermal cell into a thymic epithelial progenitor cell or a medullar thymic epithelial cell using FGF8, FGF7, FGF10, BMP4 and lithium chloride in combination, thereby inducing the differentiation of the pluripotent stem cell into the thymic epithelial progenitor cell or the medullar thymic epithelial cell. In the second induction step, a first culture is carried out under conditions in which FGF8 is added but FGF7 and FGF10 are not added to a culture medium and, subsequent to the first culture, a second culture is carried out under conditions in which FGF7 and FGF10 are added to a culture medium. |