| abstract |
The present invention relates to a method for preparing a target-specific non-antibody protein, and more particularly, relates to a method for preparing a target-specific non-antibody protein comprising the steps of: sorting non-antibody proteins having a structural complementarity with the target site of a target protein in a non-antibody protein library; calculating binding energy of the sorted non-antibody protein and the target protein; sorting a non-antibody protein having stable binding energy among the sorted non-antibody proteins; selecting an amino acid residue having high binding energy among amino acid residues in which the target protein and the selected non-antibody protein are directly contacted; and substituting the selected amino acid residue with the amino acid residue having low binding energy. In addition, the present invention relates to a target-specific non-antibody which specifically binds with EGFR (Epidermal Growth Factor Receptor) domain 2, prepared by the method and a composition for treating cancers containing the same. |