http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2010119144-A2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c96e3e0d1c1a57fc2ec7cf7fdddeeb39 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6876 |
| filingDate | 2009-04-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e305cf44344401bbdaf7b1819668f616 |
| publicationDate | 2010-10-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2010119144-A2 |
| titleOfInvention | Method for the synthesis of tail fragments of monocatenary dna from an expression vector and a universal primer, and the use thereof in hybridisation in situ |
| abstract | The invention relates to a method for obtaining monocatenary DNA probes by a first step of amplification by PCR of the selected fragment, followed by the cloning thereof in an expression vector. Once the vector has been obtained, it can be stored in host bacteria. From the vector, the insert is amplified using two primers that are complementary to the genes of the associated vector that they flank. This PCR produces a segment of dsDNA that contains the insert flanked on both ends by additional sequences of the desired length. Using this product as a template, a second PCR is carried out, using just one of the previous primers and marked oligonucleotides. As a result, monocatenary DNA probes with a tail at each end are obtained; said tail will not be able to hibridise with the target sequence but will enable a stronger signal when the total quantity of marked nucleotides is increased. |
| priorityDate | 2009-04-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 35.