| abstract |
The present invention relates to a method for analyzing particles or biomolecules in a liquid sample, comprising: detecting a signal and fluctuations in the signal from a detection volume in the sample; wherein the signal is generated from signal-generating molecules in the medium surrounding the particles or biomolecules and the fluctuations are transient reductions in the signal as the particles or biomolecules transit through the detection volume; and analyzing the detected fluctuations to obtain information about the particles or biomolecules in the liquid sample. Further, the present invention relates to a fluorescence correlation spectroscopy system comprising a laser, a zero-mode waveguide, guiding means for guiding the laser into the zero-mode waveguide, means for collecting fluorescence emission from excited molecules within the waveguide, a detector for detecting the fluorescence emission and means for autocorrelating the detected fluorescence signal, wherein the detector comprises a photomultiplier tube. Moreover, the present relation relates to the use of a fluorescence correlation spectroscopy system for analyzing molecules of interest in a sample by detecting and analyzing fluctuations in a fluorescence signal that is generated from sample molecules surrounding the molecules of interest, wherein the fluctuations are transient reductions in the detected fluorescence signal. |