| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_44ac55290f6d967effd0eed14fa73f3c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_36453782f1fe905fdb8bba6db8a994a5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f5ee73f157e45fb3594dff4862501acc
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bdbc51bd5d14d809735743b7903ed3e7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_76ba102ea9c02b6633d64bf09c5b89a4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2dcaabc343f6b3450c29bb3769f632b4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9e7ca622a3b8720203fdf92103979275 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y401-01
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y604-01001
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2830-002 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1211
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-40
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-46
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y604-01001
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y401-01
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-74
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-88
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-93 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-40 |
| filingDate |
2010-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_24350e62e35e5b3b51b3728197ee748d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4a5de51cfb1a145ffa2e82bc5bbb306f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8911b4703332f8668e700201ba9b90fe
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aaac7bdd164444e8cf64595d6ab7e788
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b414aa739c5151c8b8024181b9a5fc58
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_24b4c1a0ca8f61b095dea7c16ca5a2b0 |
| publicationDate |
2011-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2010115067-A3 |
| titleOfInvention |
Engineering the pathway for succinate production |
| abstract |
This invention relates to the biocatalysts for the efficient production of succinic acid and/or other products from renewable biological feedstocks. The biocatalysts have a very high efficiency for the growth-coupled production of succinic acid and/or other products from carbohydrate feed stocks as a result of both genetic manipulations and metabolic evolution. More specifically, certain biocatalysts of the present invention produce succinic acid at high titers and yield in mineral salts media during simple pH-controlled, batch fermentation without the addition of any exogenous genetic material. The genetic manipulations of the present invention are concerned with the energy-conserving strategies coupled with the elimination of alternative routes for NADH oxidation other than the routes for succinic acid production. The biocatalysts contain glucose-repressed gluconeogenic phosphoenol pyruvate carboxykinase (pck) derepressed by genetic modifications and a genetically-inactivated phosphotransferase system. In terms of succinic acid production efficiency, the biocatalysts of the present invention are functionally equivalent to succinate producing rumen bacteria such as Actinobacillus succinogens and Mannheimia succiniproducens with one difference that the biocatalysts are able to achieve this high level of succinic acid production in a minimal salt medium with carbohydrate source as opposed to the requirement for a rich media for succinic acid production by rumen bacteria. |
| priorityDate |
2009-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |