| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5b65747ed78f05dd9ebe107b50ce2855
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_dc01073b7aee8c88ad2173d417f0bb00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7585c33e4b935700e323985d1e3fd88a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_96ba68bf8311e0847efa3f8453c1454f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_38ab9a6a69f49cd3c099587a62ba3663 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54393
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-68 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 |
| filingDate |
2010-03-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5b73a4ce14dba9705c789b91ed045203
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_47fb378d19cda470d366f20cd8ca7700
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a9b438207feb3333dfc7291e66ee735e |
| publicationDate |
2010-09-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2010101157-A1 |
| titleOfInvention |
Method for detecting substance in biological sample |
| abstract |
Disclosed is a method for detecting a substance in a biological sample. Also disclosed is a carrier for use in the method. Further disclosed is a kit.nAn embodiment of the method comprises the following steps 1) to 4): 1) providing a carrier having biotin bound thereto, and a fusion protein of a protein capable of specifically binding to a substance to be detected and a biotin-binding protein; 2) binding the fusion protein to the carrier mentioned in step 1) to produce the carrier having the fusion protein bound thereto; 3) adding a material (a) shown below and any one of materials (b-i) and (b-ii) shown below to the carrier having the fusion protein bound thereto produced in step 2) and mixing together; and 4) detecting the substance to be detected which is bound to the protein capable of specifically binding to the substance to be detected in the fusion protein: (a) the biological sample; (b-i) a cell disruption extract prepared from a cell which is homogeneous to a host cell utilized for expressing the fusion protein mentioned in step 1), and a biotin-binding protein; and (b-ii) a cell disruption extract prepared from a cell which is homogeneous to a host cell utilized for expressing the fusion protein mentioned in step 1) and in which a biotin-binding protein is expressed by a genetically engineering technology. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108431046-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2416159-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9034590-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2416158-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9139617-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-5628793-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2013018836-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10487118-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2017115878-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2416158-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2416159-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-WO2010114031-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AU-2010232305-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10539578-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2016136130-A |
| priorityDate |
2009-03-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |