| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3b3a12c9b27c79793d6bce99c17dc493
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d55dd4da0d8f5bfa72abe0507617311d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1bb36da44ab033268582b8892a0f330b |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate |
2010-02-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7537d5961e5b9669972cb2105bba9a91
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5932c81346d773504c0d4809a87fd5ed |
| publicationDate |
2010-08-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2010091060-A1 |
| titleOfInvention |
Generation of random double strand breaks in dna using enzymes |
| abstract |
An enzyme preparation is described that includes a non-specific nuclease and a T7 Endo I mutant in a unit ratio of less than 1:200. This enzyme preparation may be used to generate double-stranded DNA fragments of a size suitable for DNA sequencing. The ends of the fragments can be readily modified as necessary to ligate adaptors or individual nucleotides to one strand of the double-stranded DNA fragments. |
| priorityDate |
2009-02-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |