| abstract |
The invention belongs to the technical field of genetic engineering of agriculture microorganisms. The invention relates to producing and using of gene cluster for thuringiensin synthesis of Bacillus thuringiensis. The gene cluster includes thuA, thuB, thuC, thuD, thuE, thuF, thuG, thu1, thu2, thu3 and thu4, about 11 genes in total. These genes have the nucleotide sequence as show in SEQ ID NO:1. And the thuringiensin biosynthetase encoded by these genes have the amino sequence as show in SEQ ID NO:2-12. The genes and proteins encoded by the genes can be used as precursor compound of pesticide library and provide new target for insecticide developing. In actual use, it would get a series of new, high efficiency and low toxic insecticide from structure remold. |