| abstract |
The present disclosure relates to simplified and improved methods for preparing aerolysin polypeptides (e.g., non-lytic variant aerolysin polypeptides), or conjugates thereof, and to use of the polypeptides and conjugates. The methods for preparation include, e.g., methods for purifying aerolysin polypeptides from a sample and methods for conjugating a heterologous moiety (e.g., a detectable label) to the polypeptides. |