| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f9a1e86160863ff07f4a5208bb95c4a2
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c06d114257182ee7efd4c696c5fdb836
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_19063ad73419844e1790857a5077d39d |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2001-4088
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-405
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-2575
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-342
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2300-0816 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L3-50273
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L3-502776 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-48
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N35-08
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-00 |
| filingDate |
2009-11-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_68761fc0e86c53d1567ce2ad395439d3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8b448c035cafeb3f224271f737765926 |
| publicationDate |
2010-05-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2010057078-A2 |
| titleOfInvention |
Method and system for generating spatially and temporally controllable concentration gradients |
| abstract |
The ability to rapidly generate concentration gradients of diffusible molecules has important applications in many chemical and biological studies. The present invention is directed to methods and systems for generating spatially and temporally controllable concentration gradients of molecules (i.e. proteins or toxins) in a portable microfluidic device. The formation of the concentration gradients can be initiated by an induced forward flow and further optimized during an induced backward flow. The forward and backward flows can be either passively induced and/or actively pumped. The centimeter-length gradients along the microfluidic channel can be spatially and temporally controlled by the backward flow. The gradient profile was stabilized by stopping the flow. In one example, a stabilized concentration gradient of a cardiac toxin, Alpha-cypermethrin, generated according to the invention was used to test the response of HL-1 cardiac cells in the microfluidic device, which correlated with toxicity data obtained from multi-well plates. The invention can be useful for biological and chemical processes that require rapid generation of concentration gradients in a portable microfluidic device. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103941022-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9651568-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107955788-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9229001-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10022696-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9855735-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9759718-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9700889-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112113904-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10228367-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10065403-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2013134741-A3 |
| priorityDate |
2008-11-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |