http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2010046918-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b597a84ce83c8fbd3e21f90409aed2fb http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6b18b65bfd9dca39cf3c679beae055de http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e0fb2070ec3c3b4c599887dd005e76e8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4c18f80aa9fe252508d428e3574d4016 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
| filingDate | 2009-10-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d27dcb8d1f5559e8a27fd7b037fb2810 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3f3b4ad9b616749ddc428d4bf31a2bdb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9a33a739d8e0da3f85b4018a36c4e49b |
| publicationDate | 2010-04-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2010046918-A1 |
| titleOfInvention | Purification of supercoiled plasmid dna |
| abstract | This invention comprises a process of purifying supercoiled plasmid DNA of purity and characteristics required for use in a DNA vaccine or in Gene Therapy or in uses where high purity supercoiled plasmid DNA is required, from a genetically transformed bacterium over-expressing the said supercoiled plasmid and the supercoiled plasmid DNA prepared by a process of this invention and products containing supercoiled DNA made by a process of this invention. The process of this invention comprises selective precipitation from a cell lyste of all DNA and endotoxin except supercoiled DNA by free metal ions. The said impurity comprising plasmid isoforms (linear, relaxed), genomic DNA, RNA, and an endotoxin from a host cell. The method is illustrated by using Cu 2+ metal ions. The said Cu 2+ metal ions were added as CuSO 4 to reach to a final concentration of at least 0.5 M. The removal of metal ions is achieved by dialysis. |
| priorityDate | 2008-10-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 28.