| abstract |
Nucleic acid probes for detecting mutations of kRas gene, liquichips and detection methods thereof are provided. The liquichips for detecting mutations of kRas gene comprise: microspheres coated with wild-type and mutant probes, each of which is modified with amino group at 5’-terminal, specific for kRas gene codons 12, 13 and/or 61, and primers for amplifying the target sequence biotin-labeled at the terminal which are enriched with mutant sites of kRas gene codons 12, 13 and/or 61 to be detected. The detection methods are rapid and correct, and the processes of the methods are easy to perform. The methods enable detecting multiple mutations in one well simultaneously, thus can greatly improve the efficiency of detection. The liquichip can be used to detect mutations of kRas as assistance to early diagnosis of pancreatic cancer, and can be used to prognose efficiency of the molecular targeted therapy to choose right medicine accurately clinically and avoiding economic loss and time loss caused by unnecessary treatment. |