http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2010045326-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4edd49b07310c18a4aa364e7c28c5983 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
| filingDate | 2009-10-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_63bfc0fa3bf624cbead597bd39bd6abc |
| publicationDate | 2010-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2010045326-A1 |
| titleOfInvention | In vitro recombination methods using 5' exonuclease |
| abstract | A method for homologous end cloning using a 5'-exonuclease is disclosed. The method entails generating a recombinant DNA molecule using 5' exonuclease digestion of ONA molecules, exonuclease inactivation, and annealing of 3' overhangs to form a annealed complex. The method may further comprise extension of 3' ends to fill gaps corresponding to exonuclease removal to form substantially non-gapped annealed complex. Complexes formed can be transformed into a host cell without further in vitro processing. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10787702-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10415082-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021064115-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10087483-B2 |
| priorityDate | 2008-10-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 28.