| abstract |
The invention relates to a fluorescent emulsion, to its uses and to labelling reagents comprising it. The fluorescent emulsion of the invention is of the oil-in-water type, comprising at least one aqueous continuous phase in which droplets of at least one oil phase are dispersed, said oil phase droplets being stabilized by a surfactant layer, characterized in that it comprises at least one pair of labels, differing from one another, formed from a donor fluorescent label that absorbs at a wavelength λ 1 and emits at a wavelength λ 2 different from λ 1 , and an acceptor label that absorbs at the emission wavelength λ 2 of the donor fluorescent label; in that the donor fluorescent label and the acceptor label are kept close together by the encapsulation of one of them in the oil phase droplets and either by linking the other of them to the oil phase droplet/aqueous phase interface, or by the encapsulation of the other of them in the oil phase droplets; and in that it comprises molecules of at least one amphiphilic surfactant and at least one solubilizing lipid. The fluorescent emulsion of the invention is applicable in the field of optical fluorescence imaging, in particular optical fluorescence biomedical imaging. |