| abstract |
The invention relates to methods for the enzymatic hydrolysis of materials. More specifically, the invention relates to a method for hydrolysing a polysaccharide, the method comprising the steps of: (i) contacting the polysaccharide with mesophilic and thermophilic hydrolytic enzymes to produce a reaction mixture, (ii) incubating the reaction mixture at a temperature suitable for activity of the mesophilic hydrolytic enzymes, and (iii) incubating the reaction mixture at a temperature suitable for activity of the thermophilic hydrolytic enzymes, wherein the polysaccharide is hydrolysed by said mesophilic and thermophilic hydrolytic enzymes to produce two or more polysaccharide fragments. The invention further relates to sugar fragments produced by the enzymatic hydrolysis methods. |